The relationship between morphology and disaccharidase activity in ischemia- reperfusion injured intestine.
نویسندگان
چکیده
BACKGROUND Questions regarding functional markers characterizing injured intestines remain unanswered. Brush border disaccharidases are crucial for the functioning of the intestines. AIMS The study was designed to assess changes in disaccharidase activity (DA) following intestinal injury and to compare them with morphological changes. METHODS Wistar rats, randomly divided into six experimental groups (each n = 6), were subjected to different ischemic/reperfusion injury. One-hour mesenteric ischemia followed by reperfusion for 0, 1, 2, 4, 12 or 24 hours was induced. As a control group sham-operated animals were used (n = 6). Intestine morphology was evaluated using histopathological injury index (HII) and goblet cell (GC) detection. DA (sucrase and maltase) was studied in mucosal scrape or in entire intestinal wall samples. RESULTS Moderate morphological damage (HII, GC) after mesenteric ischemia was detected. Deepening of the injury was found during reperfusion with a maximum after two hours. Improved morphology with longer reperfusion confirmed reversible damage with almost normal mucosal structure after 24 hours of reperfusion. Similar pattern was observed when DA was measured. The lowest activity was detected after 2 hours of reperfusion followed by increasing activity in the subsequent reperfusion periods. Physiological values after 24 hours of reperfusion were seen only in samples of entire intestinal wall. CONCLUSIONS Significant changes in intestinal DA were observed after intestinal ischemia/reperfusion injury. A similar pattern was seen for morphological characteristics. Although based on microscopic survey the intestine seems to be fairly regenerated, some functional limitation is expected to persist.
منابع مشابه
The Protective Effect of Orally Administered Amlodipine against Intestinal Ischemia-Reperfusion Injury in Rats
Objective- This study investigated the effect of amlodipine on intestinal ischemia-reperfusion injury in ratsDesign-Experimental studyAnimals-Fifteen male Sprague-Dawly rats weighing 200-220gProcedure- Rats were randomly divided into 3 groups: IR group (operation with clamping), sham group (operation without clamping), and IRA group (operation with clamping and 5mg/kg amlodipi...
متن کاملProtective Effect of Trigonella foenum graecum (fenugreek) Seed Extract on Experimental Intestinal Ischemia/Reperfusion Injury in Rats
Objective- This study was performed to evaluate whether Trigonella foenum graecum (fenugreek) seeds extract has any protective activity in intestinal ischemia/reperfusion injury in rat. Design- Experimental study. Animals- 20 male Wistar albino rats. Procedures- Four groups of rats were included in this study. Group I (I/R) underwent ischemia-reperfusion (I/R) of the intestine (45 min of isc...
متن کاملBiological and Histopathological Investigations of Moclobemide on Injured Ovarian Tissue Following Induction of Ischemia-Reperfusion in Rats
متن کامل
The preventive effects of dexmedetomidine against intestinal ischemia-reperfusion injury in Wistar rats
Objective(s): Intestinal ischemia-reperfusion is a major problem, which may lead to multiorgan failure and death. The aim of this study was to evaluate the protective effects of dexmedetomidine on cell proliferation, antioxidant system, cell death, and structural integrity in intestinal injury induced by ischemia-reperfusion in rats. Materials and Methods: Animals were randomized into three gro...
متن کاملThe effects of adenosine injection after of brain ischemia reperfusion injury on gene expression of NF-kB/p65 and activity level of ROS in male Wistar rats
Background: Unit of p65 is one of the subunits of NF-κB and its phosphorylation by stress oxidative causes activation of NF-κB. The aim of present study was to investigate the effects of adenosine injection after brain ischemia reperfusion injury on gene expression of NF-κB /p65 and Reactive Oxygen Species (ROS) in hippocampus tissue of male wistar rats. Methods: 40 male wistar rats were rando...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Acta biochimica Polonica
دوره 59 4 شماره
صفحات -
تاریخ انتشار 2012